Functional Assays

The Chemi-Verse™ PI3 Kinase (p120γ) Kinase Assay Kit is designed to measure PI3 (phosphoinositide 3) kinase (p120γ) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PI3 kinase (p120γ), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ SIK1 Kinase Assay Kit is designed to measure SIK1 (salt-inducible kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant SIK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ SIK2 Kinase Assay Kit is designed to measure SIK2 (salt inducible kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant SIK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ PI3 Kinase (p110α/p85α) Kinase Assay Kit is designed to measure kinase activity of the PI3 kinase (p110α/p85α) complex for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PI3 kinase (p110α/p85α), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ PI3 Kinase p110α (E545K)/p85α Kinase Assay Kit is designed to measure the kinase activity of the complex PI3 (phosphoinositide 3) kinase consisting of mutant p110α (E545K) and p85α for screening and profiling applications, using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant human complex PI3 Kinase p110α (E545K)/p85α kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions. 

The Cathepsin E Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin E for screening and profiling applications. The Cathepsin E assay kit comes in a convenient 96-well or 384-well format, with enough recombinant human Cathepsin E (amino acids 18-396), its substrate, and Cathepsin buffer for 96 or 384 reactions. This kit includes inhibitor Pepstatin A as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The Cathepsin E Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin E for screening and profiling applications. The Cathepsin E assay kit comes in a convenient 96-well or 384-well format, with enough recombinant human Cathepsin E (amino acids 18-396), its substrate, and Cathepsin buffer for 96 or 384 reactions. This kit includes inhibitor Pepstatin A as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.

The PADI4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure the enzymatic activity of PADI4 (protein-arginine deiminase type-4) for screening and profiling applications. This assay uses o-phthalaldehyde and DTT (dithiothreitol) as detection reagents. The assay kit comes in a convenient 96-well format, with enough purified recombinant PADI4, BAEE (Nα-Benzoyl-L-arginine ethyl ester hydrochloride), EDTA (Ethylenediaminetetraacetic acid), o-phthalaldehyde, and assay buffer for 100 reactions.

The Chemi-Verse™ PI3 Kinase P110α (E542K)/P85α Kinase Assay Kit is designed to measure the kinase activity of the complex PI3 (phosphoinositide 3) kinase consisting of mutant P110α (E542K) and P85α for screening and profiling applications, using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant human complex PI3 kinase p110α (E542K)/p85α kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.