Functional Assays

The Cathepsin L Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin L for screening and profiling applications. The Cathepsin L assay kit comes in a convenient 96-well or 384-well format, with purified Cathepsin L (amino acids 18-333), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.  

The CDK9/CyclinT Kinase Assay Kit is designed to measure CDK9/CyclinT kinase activity for screening and profiling applications using Kinase-Glo™ Max as a detection reagent. The assay kit comes in a convenient 96-well or 384-well format, with enough purified recombinant CDK9/CyclinT kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The CDK9/CyclinT Kinase Assay Kit is designed to measure CDK9/CyclinT kinase activity for screening and profiling applications using Kinase-Glo™ Max as a detection reagent. The assay kit comes in a convenient 96-well or 384-well format, with enough purified recombinant CDK9/CyclinT kinase, kinase substrate, ATP, and kinase assay buffer for 400 enzyme reactions.

The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor. PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMP is quenched. Proteolysis releases AMP and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.

The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor. PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMP is quenched. Proteolysis releases AMP and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.

The DNA Polymerase γ Assay Kit is a fluorogenic assay designed to measure DNA Polymerase γ activity for screening and profiling applications, using AccuGreen™ as detection reagent. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase γ enzyme, DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.                                                                                                                                  Note: AccuGreen™ High Sensitivity dsDNA Quantitation Kit is sold separately by Biotium. DNA Polymerase γ uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecule (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can quantified by the addition of the fluorescent DNA-binding dye which allows to distinguish dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound

The DNA Polymerase β Assay Kit is a fluorogenic assay designed to measure DNA Polymerase β activity for screening and profiling applications, using AccuGreen™ as detection reagent. The kit comes in a convenient 96-well format, with enough recombinant Polymerase β enzyme, DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions. Note: AccuGreen™ High Sensitivity dsDNA Quantitation Kit is sold separately by Biotium. DNA Polymerase β uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecule (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can quantified by the addition of the fluorescent DNA-binding dye which allows to distinguish dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.

The Chemi-Verse™ AMPK (A1/B1/G1) Kinase Assay Kit is designed to measure AMPK ((AMP-activated protein kinase) (A1/B1/G1)) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant AMPK (A1/B1/G1) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ PI3 Kinase P110α(H1047R)/P85α Kinase Assay Kit is designed to measure the kinase activity of complex PI3 (phosphoinositide 3) kinase consisting of mutant P110α (H1047R) and P85α for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant human complex PI3 kinase P110α(H1047R)/P85α kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.