Page 22 - Metabolism

Expression systems - removing their contaminants

CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and development

Functionality and viability of primary cells can be impaired by incorrect thawing procedures, storage or culture conditions. It's generally admitted that applying the same protocols as for cell lines

Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low efficiency.

Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications

A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA enhance

PD-1:PD:-L1 immune checkpoint pathway targeting immunotherapies have shown great potential for many cancer patients. Recently, The FDA has granted accelerated approval to the immunotherapy drug pembrolizumab

Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated

Gastrointestinal (GI) primary cells represent a powerful approach for the in vitro study of the physiopathology of this unique tissue. Researchers have now identified intimate interactions between GI

Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.

The TrueBlot® reagents

In the last few decades, in the field of Drug Discovery, Immunotherapy has gained more and more importance for the treatment of various diseases and in particular for certain types of cancer. Based on

Undoubtedly, producing and purifying a protein is an art form.

Indeed, each recombinant protein (rec. protein) to be produced in vitro has specific characteristics and singularities, which makes its production

By mimicking in-vivo environments, 3D cell culture models appear nowadays as the best in-vitro study model to work in an in-vivo like study model and to obtain more physiologically relevant data and proof

If your research is related to obesity and adipose tissue, you may be interested by the selection I have performed below of immunoassays (including ELISA tests and antibody arrays), as well as primary