Recombinant protein production and protein purification raises the tricky question of host cell proteins (HCPs). To monitor the purification level of your protein of interest, the HCPs must be quantified. Of course, the quality and the optimisation of purification depends upon it. Here, we’ll take a look at an alternative to the gold standard, offering a way to detect more HCPs present in the production. So, are you ready for higher grade detection?
How about 25% more coverage?
The Canopy process of affinity purification of antibodies is very different from the leading competitor, giving us more sensitive antibodies that recognize a wider range of host cell proteins.
Canopy’s CHO HCP antibody was tested under similar conditions as competitor “C’s” CHO HCP antibody using 2D western blot analysis.
Results indicate Canopy’s antibody has 25% more coverage. Berkelman, T., Harbers, A., and Bandhakavi, S. Biorad Bulletin 6393. Wang, X. Unpublished data ang, X. Unpublished data.
2-8 fold more HCPs
The amount of HCP present in 3 different DSs (therapeutic antibodies, produced by CHO) was assessed by both the Canopy CHO HCP kit and the Competitor “C” kit. Canopy’s antibody detected 2-8 fold more HCPs. Fas, S. BioPharmaceutical Emerging Best Practices Association. 2014
Intra-assay validation
Canopy’s CHO HCP antibody exhibited less intra-assay variation than the antibody bt Competitor C.
Lot-to-lot consistency
Lot-to-lot variation was assessed using 3 different lots against 90, 30 and 10ng/ml. Lot-to-lot variation was negligible for the Canopy antibody.
Find your new HCP detection kit
Are you convinced? Take a look at the kits below:
- CHO Host Cell Proteins ELISA kit
- E. coli Host Cell Proteins ELISA kit
- Pichia Host Cell Proteins ELISA kit
We can also offer protein purification and HCP detection as a service, in our own European based laboratories in France – get in touch to learn more through the form below.
