Cells

Human Motor Neurons (iPSC-derived, TDP-43 mutation, M337V, ISO)

Human Motor Neurons (iPSC-derived, TDP-43 mutation, M337V, ISO)

Please note this product may be subject to fees, we invite you to contact your local office. ADAR1 Responsive Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to respond to ADAR1 enzymatic activity. These cells were engineered to express an ADAR1 reporter construct comprised of an ADAR1 hairpin target with a stop codon (UAG) susceptible to ADAR1-mediated editing to tryptophan (UUG), located upstream of a firefly luciferase reporter This cell line has been validated by comparing reporter activation after transfection with ADAR1 and ADAR2. This cell line has been used for the development of the constitutive ADAR1 expressing ADAR1 Activity Luciferase Reporter HEK293 Cell Line (#82239).

Please note this product may be subject to fees, we invite you to contact your local office. ADAR1 Activity Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor the activity of ADAR1 (adenosine deaminase acting on RNA) enzyme. These cells were engineered to express ADAR1 (NM_001111.5) and an ADAR1 reporter construct comprised of an ADAR1 hairpin target with a stop codon (UAG) susceptible to ADAR1-mediated editing to tryptophan (UUG), located upstream of a firefly luciferase reporter. This cell line has been validated by comparing reporter activation to ADAR1 Responsive Luciferase Reporter HEK293 Cell Line (#82238) and in response to treatment with ADAR1 siRNA.

293 cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 106 cells/100µl in lysis buffer.

NIH3T3 cells were treated with Pervanadate. Solubilize cells at 4 x 106 cells/100µl in cell lysis buffer.

COS cells were treated with Forskolin. Solubilize cells at 4 x 106 cells/100µl in cell lysis buffer.

H1993 cells were cultured at 37°C to reach 85% confluence. Cells were solubilzed at 4 x 106 cells/100µl in lysis buffer.

Hela cells were treated with IGF. Cells were solubilzed at 4 x 106 cells/100µl in cell lysis buffer.