Functional Assays

96-well Rapid Neutralizing Antibody Assay Kit for SARS-CoV-2 w/ Ready to use target cells - (Variant) Kit Includes: 5 mL of Ha-CoV-2 Pseudovirus Particles 1 Tube of Ready-To-Use Target Cells (5 Million Cells/Tube) 50 µL of Standard SARS-CoV-2 Neutralizing Antibody 15 mL of VBI Infection Medium 2 mL of Lysis Buffer 3.5 mL of Firefly Luciferase Assay Buffer

Low-Speed Viral Concentration kit, 250 ml

Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL17 (C-C motif chemokine 17; CC chemokine TARC) in cell culture supernatants. The kit does not include a recombinant human CCL17/TARC standard, which must be purchased separately from R&D Systems® (catalog number 364-DN).

The PDE3A TR-FRET Assay Kit is designed to provide fast and easy identification of inhibitors of PDE3A (phosphodiesterase 3A) using Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET). The PDE3A TR-FRET Assay Kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PDE3A (amino acids 669-end) enzyme, fluorescently labeled PDE substrate (cAMP), binding agent, and PDE assay buffer for 100 or 384 enzyme reactions. The reaction uses a fluorescein-conjugated (FAM) cyclic monophosphate nucleotide.  Phosphodiesterase PDE3A catalyzes the hydrolysis of the phosphodiester bond in the cyclic monophosphate nucleotide, releasing the phosphate group for binding. The phosphate group binds to a "Binding Agent" (BA) that is recognized by terbium-labeled donor beads. This results in energy transfer from the terbium to FAM, which emits a fluorescent signal at 520 nm. If unbound to the phosphate group, the terbium-labeled beads emit at λ=490 nm. The fluorescent int

The SARM1 Fluorogenic Assay Kit (Hydrolase Activity) is designed to measure NAD⁺ cleavage activity in screening and profiling applications. The SARM1 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant human SARM1 enzyme, its substrate N6-etheno-NAD (e-NAD), and SARM1 assay buffer for 100 or 400 enzyme reactions. In addition, the kit includes a SARM1 inhibitor (DSRM-3716) for use as a control inhibitor. Hydrolase activity of SARM1 is measured by following the hydrolysis of Etheno-NAD to form Etheno-ADPR + nicotinamide. Etheno-NAD is not fluorescent due to internal quenching. Upon hydrolysis by SARM1, nicotinamide is released, leading to an increase in fluorescence signal directly proportional to the enzymatic activity.

The DCAF11 Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure DCAF11 auto-ubiquitination activity in a homogeneous 384-reaction format. It utilizes Europium-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF11. As a homogenous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses. <img style="display: block; margin-left: auto; margin-right: auto;" src="{{media url="wysiwyg/ubiquitin/78542.png"}}" alt="" width="1059" height="225" /> Figure 1. DCAF11 Intrachain TR-FRET Assay Kit Schematic.

The EPHA4 Kinase Assay Kit is designed to measure EPHA4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

The Von Hippel-Lindau tumor suppressor (VHL) Binding Assay Kit is a homogeneous assay which measures the binding of VHL to the high-affinity VHL fluorescent probe (BDY FL VH032) using Fluorescence Polarization (FP). In addition, the kit includes the VHL inhibitor VH298 for use as an inhibitor control. This assay takes advantage of the fluorescent substrate BDY FL VH032. Using this kit, only one step on a 96-well plate is required. The BDY FL VH032 is incubated with the VHL complex in the presence of a compound of interest to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

The P70S6K (S6K1) Kinase Assay Kit is designed to measure P70S6K (S6K1) kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.