Bioconjugation Kits

MagicLink™ technology is the third generation of protein crosslinking technology and has been engineered to allow rapid conjugation of all classes of biomolecules, such as protein, antibody, enzyme, nucleic acid, etc. The proprietary chemistry is based on instant reaction between MAGIC activated biomolecule and LINK activated biomolecule. It is featured as instant conjugation reaction, almost quantitively yield, and most stable linkage on the market. In some cases, the exact number of LINK per biomolecule is need. 650-MAGIC offers scientists a way to estimate how many LINK moieties were successfully coupled to their molecules or proteins of interest. In pH 7-9.0, 650-MAGIC instantly reacts with LINK-activated protein to form 650 conjugates. By following the easy protocol below, scientists can calculate the ratio of link/ protein which is the ratio of dye per protein.

MagicLink™ technology is the third generation of protein crosslinking technology and has been engineered to allow rapid conjugation of all classes of biomolecules, such as protein, antibody, enzyme, nucleic acid, etc. The proprietary chemistry is based on instant reaction between MAGIC activated biomolecule and LINK activated biomolecule. It is featured as instant conjugation reaction, almost quantitively yield, and most stable linkage on the market. In some cases, the exact number of LINK per biomolecule is need. 650-MAGIC offers scientists a way to estimate how many LINK moieties were successfully coupled to their molecules or proteins of interest. In pH 7-9.0, 650-MAGIC instantly reacts with LINK-activated protein to form 650 conjugates. By following the easy protocol below, scientists can calculate the ratio of link/ protein which is the ratio of dye per protein.

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

MagicBrite ECL DuraPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects high femo gram amounts of antigen with stable and long light output (5 to 24 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

MagicBrite ECL DuraPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects high femo gram amounts of antigen with stable and long light output (5 to 24 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

MagicLink™ Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get the conjuga

MagicLink™ Site Specific Biotinylation Kits provide optimized reagents for biotinylating antibodies. Biotin is a small, naturally occurring vitamin that binds with high affinity to avidin and avidin-like proteins. Biotinylated antibodies typically retain biological activity because the biotin group is relatively small. Current bintinylation methods, such as amine-NHS ester and converting amine to thiol and then use maleimide chemistry, will produce random biotin on the antibody. In some cases, labeling in some Fab areas could block antigens binding. With site specific biotinylation chemistry, biotin linkers are inserted to thiol-bridge locations and do not affect antibody-antigen binding (Fig.1). It will help increase the sensitivity of bio assay such as ELISA, Western Blot, etc. This conjugation reaction is simple, site-specific, and efficient. These kits include biotin linker, reaction buffer, protein concentrator, DMSO, and TCEP. The user can follow easy protocol and get the conjuga