Functional Assays

The Chemi-Verse™ PARP7 Assay Kit is an ELISA-type chemiluminescent assay designed to measure PARP7 enzymatic activity for screening and profiling applications. PARP7 catalyzes the NAD-dependent ADP-ribosylation of histones. This 96-well or 384-well format assay kit contains sufficient amounts of purified PARP7 enzyme (amino-acids 400- 657), histone mixture, and PARP assay buffer for 100 or 400 enzyme reactions. The Chemi-Verse™ PARP7 Assay Kit takes advantage of a highly specific ADP-ribose binding reagent. First, histone proteins are coated on a 384-well plate. Next, an NAD⁺ substrate is incubated with the PARP7 enzyme in an optimized assay buffer. Finally, the plate is treated with the ADP-ribose specific binding reagent and secondary HRP-conjugated antibody, followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. <img src="{{media url="wysiwyg/PARPs/thumbnail_HS-PARP7-diagram-large.jpg"}}" alt=""

The Fibroblast growth factor receptor 4 (FGFR4) Kinase Assay Kit is designed to measure FGFR4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

The C-terminal Src kinase (CSK) Kinase Assay Kit is designed to measure CSK kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

The transforming growth factor beta receptor (TGFβR1, also known as ALK5) Kinase Assay Kit is designed to measure the serine/threonine activity of TGFβR1 (ALK5) for screening and profiling applications using ADP-Glo^® as a detection reagent. 

The CBL-B-driven SRC ubiquitination intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination. <img style="display: block; margin-left: auto; margin-right: auto;" src="{{media url="wysiwyg/ubiquitin/78820.png"}}" alt="" width="900" height="220" /> Figure 1: CBL-B-driven SRC ubiquitination intrachain TR-FRET Assay Kit schematic.

The CBL-B-driven AXL ubiquitination intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination. <img src="{{media url="wysiwyg/ubiquitin/78821.png"}}" alt="" width="850" height="207" /> Figure 1: CBL-B-driven AXL ubiquitination intrachain TR-FRET Assay Kit schematic.

The C-CBL-driven SRC Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination. <img src="{{media url="wysiwyg/ubiquitin/78822.png"}}" alt="" width="850" height="207" /> Figure 1: C-CBL-driven SRC ubiquitination intrachain TR-FRET Assay Kit schematic.

The C-CBL-driven AXL Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination. <img src="{{media url="wysiwyg/ubiquitin/78823.png"}}" alt="" width="850" height="207" /> Figure 1: C-CBL-driven AXL ubiquitination intrachain TR-FRET Assay Kit schematic.

The FGL1:LAG3 Inhibitor Screening Assay is an AlphaLISA®-based assay designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 384-reaction format. The assay is straightforward: first, FGL1 and LAG3 are incubated with or without a compound of interest. Then, acceptor and donor beads are added to the reaction, followed by reading the Alpha-counts. <img src="{{media url="wysiwyg/Imtx/thumbnail_78824_LAG3-LGL1_illustration.png"}}" alt="" width="900" height="253" /> Illustration of the assay principle.<br />FGL1 contains a His tag recognized by the Nickel chelate acceptor bead, whereas LAG3 is biotinylated, allowing its binding to the streptavidin-coated acceptor bead. Interaction between FGL1 and its receptor LAG3 brings the donor and acceptor beads in proximity. A singlet oxygen generated upon excitation of the donor bead leads to the excitation of the acceptor bead, which emits light. Light emission in the assay is proportional to the level of interaction. AlphaLISA™