Functional Assays

The Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate.Assay sensitivity: 25 pmolSample Type: tissue homogenates cell lysatesSample Volume: 50 µL/wellProduct BackgroundThe  Acid Sphingomyelinase Assay Kit utilizes a fluorogenic substrate that is specific for acid sphingomyelinase to provide a sensitive and homogenous method to measure the activity of aSMase in vitro. The kit provides all necessary reagents to measure the acid sphingomyelinase activity of 40 samples ran in duplicate.Sphingomyelinases catalyzes the hydrolysis of sphingomyelin into ceramide and phosphoryl choline; and is involved in programmed cell death (apoptosis) cell differentiation and cell proliferation. Sphingomyelinases are classified into five categories: acid sphingomyelinase (aSMase) secretory sphingomyelinase (sSMase) neutral Mg 2 +/- dependent sphingomyelinases (nSMase) neutral

Shuttle PIP kits are designed for researchers interested in visualizing and localizing intracellular phosphoinositides. These kits contain fluorescent and non-fluorescent phosphoinositides carriers and fluorescent-labeled carriers. Two-color studies are possible using BODIPY® FL labeled PIPs and tetramethylrhodamine (TMR)-labeled carriers.Kit ContentsPhosphoinositidesPI(3 4)P2diC16BODIPY FL-PI(3 4)P2CarriersNeomycin SulfateNeomycin-TMRHistone H1Histone H1-TMRCarrier 3

Please note this product may be subject to fees, we invite you to contact your local office. The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one

The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Uric Acid Assay Kit is a quantitative colorimetric uric acid determination at 590 nm.

Isolate up to 200 µg transfection grade, low-endotoxin plasmid DNA (<1EU/µg) from 15-50 mL bacterial cultures using syringe filters for lysate clearance and midi spin columns

Isolate up to 200 µg transfection grade, low-endotoxin plasmid DNA (<1EU/µg) from 15-50 mL bacterial cultures using syringe filters for lysate clearance and midi spin columns

Isolate low endotoxin (<1EU/µg) plasmid DNA from up to 200 mL bacterial cultures using spin maxi columns