Functional Assays

This kit is used for the fast quantification of Trastuzumab/Herceptin® (Monkey) in serum,plasma and cell supernatant samples. (Homogenous Assays, no wash step, quantitative assay)

The Aldosterone Urine Supplementary kit is a reagent set intended to be used for the manual preparation of urine samples to be used with Aldosterone ELISA (KA1883).

The DNA Polymerase θ Assay Kit is a fluorogenic assay designed to measure DNA Polymerase θ activity for screening and profiling applications, utilizing the fluorescent nucleic acid dye GroovyGreen™ for the quantitation of double-stranded DNA (dsDNA) products. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase θ enzyme (amino acids 1792-2590), DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.  DNA Polymerase θ uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecules (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.  

A kinetic biosensor assay for arrestin recruitment converts conformational changes in arrestin-3 (β-arrestin-2) to changes in fluorescence intensity. This kit detects arrestin recruitment at the unmodified µ-Opioid Receptor (MOR). The Borealis Arrestin Sensor, and MOR, are packaged in a BacMam viral vector for easy delivery to most cell types. Validated for use on fluorescence imaging systems and automated fluorescence plate readers.

Sircol 2.0 is the newest member of Biocolor's family of collagen kits, designed to simplify quantative measurement of soluble collagens from in-vivo & in-vitro samples. It provides enhanced sensitivity and specificity for accurate collagen measurement, surpassing the performance of our previous soluble collagen kit.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.

The Cathepsin K Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin K for screening and profiling applications. The Cathepsin K assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin K, its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched flourogenic substrate.Proteolysis releases the highly flourescent Mca from the quencher. Flourescence intensity increases proportionally to the activity og the protease.

The PARG Fluorogenic Assay Kit is a high-throughput, homogeneous 96-well or 384-well assay designed to measure the hydrolase activity of Poly (ADP-ribose) glycohydrolase (PARG) for screening and profiling applications, using a simple and straightforward fluorogenic assay. The PARG Fluorogenic Assay Kit contains enough purified recombinant PARG enzyme, substrate, and assay buffer for 100 or 400 enzyme reactions. <p style="text-align: center;"><img src="{{media url="wysiwyg/PARPs/78858.png"}}" alt="" width="388" height="188" /> Figure 1: Illustration of the assay principle.<br />PARG is incubated with a fluorogenic ADP-ribose substrate in which the fluorophore is quenched by the presence of the ribose. PARG-mediated hydrolysis of the substrate between the ribose and the fluorochrome releases fluorescence that can be detected at λ=502 nm (excitation at λ=385 nm). Fluorescence intensity is directly proportional to PARG hydrolase activity. 

The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well 384-well format, with enough purified Cathepsin S (amino acids 17-331), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.  The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.