Cell-based Assays

The RayBiotech urea nitrogen (BUN) colorimetric assay kit provides a simple, reproducible, and sensitive tool for detecting urea nitrogen in plasma, serum, cell lysates, urine and other biological liquid samples.

The RayBiotech urea nitrogen (BUN) colorimetric assay kit provides a simple, reproducible, and sensitive tool for detecting urea nitrogen in plasma, serum, cell lysates, urine and other biological liquid samples.

The RayBiotech urea nitrogen (BUN) colorimetric assay kit provides a simple, reproducible, and sensitive tool for detecting urea nitrogen in plasma, serum, cell lysates, urine and other biological liquid samples.

The RayBio® Calcium Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for measuring calcium concentration in plasma, serum, cell lysates, urine and other biological liquid samples.

The RayBiotech Pyruvate Assay Kit provides a simple, reproducible, and sensitive tool of measuring pyruvate concentration in plasma, serum, cell lysates, saliva, urine and other biological liquid samples.

The RayBiotech Pyruvate Assay Kit provides a simple, reproducible, and sensitive tool of measuring pyruvate concentration in plasma, serum, cell lysates, saliva, urine and other biological liquid samples.

Glucose-6-phosphate (6PG), also known as Glucose 6-phosphate, is an intermediate product of glycolysis and pentose phosphate pathway, which is widely found in animals, plants and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to form glucose-6-phosphate, which is then catalyzed by phosphate glucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis. However, in the pentose phosphate pathway, 6PG is its first substrate, and this process is also the main way to generate NADPH. In addition, 6PG can also be converted to glycogen or starch and stored. Glucose-6-phosphate dehydrogenase catalyzes the formation of glucose-6-phosphate and NADPH from 6PG and NADP+. NADPH can make WST-8 orange-yellow under the action of 1-mPMS, and the content of 6PG can be calculated by measuring the absorbance value at 450 nm.

GCDH (EC 1.1.1.47) catalyzes D-glucose and NAD(P) to form D-Gluconic acid and NAD(P)H, which are mainly found in the liver of many microorganisms and higher animals. The use of GCDH in the production of oligofructose not only removes glucose from oligofructose and increases its content, but also generates gluconic acid that combines with calcium ions to form calcium gluconate, which is an ideal calcium supplement. Therefore, GCDH has become an ideal enzyme for preparing high content oligofructose. GCDH catalyzes the formation of d-Gluconic acid and NADH from D-glucose and NAD. The activity of glucose dehydrogenase can be reflected by the change of absorbance value of NADH at 340 nm.

Methylcitrate synthase (MCS) exists widely in the mitochondrial matrix of animals, plants, microorganisms and cultured cells, and participates in the regulation of tricarboxylic acid cycle together with citrate synthase (CS). CheKineTM Micro Methylcitrate Synthase (MCS) Activity Assay Kit can be used to detect biological samples such as animal and plant tissues,Cells, Serum orother Liquid samples. In this kit, MCS catalyzes propionyl CoA and oxaloacetic acid to produce methyl citric acid, which further hydrolyzes to methyl citric acid, which promotes the conversion of colorless DTNB to yellow TNB with characteristic absorbance at 412 nm.