Scientific Library
Flow cytometry intracellular staining
Intracellular Flow Cytometry enables the identification and analysis of signaling and functional markers within cells from samples containing heterogeneous cell populations. Many cell types can be thus
DNA repair antibodies validated by MD Anderson
A series of publications (1, 2) in the past months has raised the never-ending debate on commercial antibodies and their validity in a given experiment. At tebu-bio, having sold antibodies for more than
DyLight™ will be your routine fluorescent dye
Rockland Immunochemicals have developed a suite of fluorescent dyes (DyLight™) with unique fluorescence intensity and photostability. Here, we take a look at how DyLight™ dyes have all the characteristics and potential to become your daily fluorescent dye conjugates.
#1- What makes DyLight™ dyes unique?
DyLight™ conjugates are ideal substitutes for IRDye® products as far as experimental results and budgets are concerned. For example, DyLight™ 680 and DyLight™ 800 products are an excellent alternative to Infra-Red and Near Infra-Red IRDye® products.
#2- Are DyLight™ dyes compatible with common fluorescence instrumentation?
#3- What range of applications do DyLight™ dyes cover?
DyLight™ conjugated antibodies are ideal for TrueBlot® IP / Western blotting, Multiplex imaging, Flow cytometry, IF, ELISA, High Content screening, High resolution microscopy...
We've noted that these are some of the most popular Dylight™ products:
- Anti-MOUSE IgG (H&L) (DONKEY) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-745-124)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-145-121)
- Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039611-145-122)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 680 Conjugated Pre-Adsorbed (cat. nr 039610-144-121)
Well... has this given you any ideas, would you like to learn more or try Rockland's DyLight™ fluorescent conjugated products?
Get in touch with tebu-bio's experts, or leave a message below.
Following your proteins - anti-tag antibodies (I)
When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not
Antibody purification troubleshooting tips
Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade data.
3 tips for IHC troubleshooting
The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative
Obtain clear Western Blot and Immunoprecipitation results
Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.
Breast Cancer - Popular validated primary antibodies for research and biomarker discovery
Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the
Anti-Tag antibodies you can rely on: a focus on GFP and RFP
My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely
Immunoassay development: reliable sources of quality reagents
We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years,