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If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?

Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,

Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so there shouldn't be any need to look for alternatives

Following our series of posts on the role of exosomes in cancer (TME, signaling), let's have a look at Neurobiology today. Because exosomes are everywhere (even in beer!).

Whatever the research domain (oncology, cardiovascular diseases, Diabetis/obesity, inflammation, autoimmune disorders...), phosphorylation study is essential when looking for activation of cell signaling

What are metabolites?

Microorganisms live in a world of chemical signals. They use small molecular weight compounds, known as metabolites, to regulate their own growth and development,

The involvement of Prostaglandin E2 (PGE2) in cancer development has already been described (see the post "Tumour

Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing

To dilute or not to dilute biological samples? And if yes, how much? That's the question!

Following our post on whether samples should be pooled or not when performing studies for

Unfortunately, and still in the XXIst century, micronutrient (MN) malnutrition is a problem in all countries, in all segments of society. While part of the world starves, another part is obese. And a balanced

Use of human samples including peripheral blood mononuclear cells (PBMCs) in drug discovery is critical for increasing the chances of success for a small molecule screen hit. A review based on recent published

In previous blogs, I invited you to join me in exploring the relevance of the following pathways:

Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.

Case # 1 - ELISA or simplex