Page 33 - Cosmetology
Live Cell Imaging: User results with SiR-Actin & SiR-Tubulin
For one year now, SiR-Actin and SiR-Tubulin, the second generation of tools to stain actin and tubulin in
STAT3 signaling effects on IL-15 derived dendritic cells
The role of STAT3 signaling in the activity of dendritic cells (DC) has been recently revealed in a publication by Okada et al.
Intracellular Calcium measurement with Fluo-8
G Protein Coupled Receptors (GPCR) and ion channels are important targets in drug development. Both target classes represent membrane proteins which require convenient and robust cell-based assays for
How to modulate ion channels
Ion channels play an important role in perception and transmission of pain sensations. Furthermore, dysfunctions of certain ion channels are involved in neuronal disorders, such as epilepsy, Alzheimer's
Ion channel over expressing cell lines for inhibitory screening
In a recent post on Venomous toxin ion channel modulators, we looked at toxin derived peptides to manipulate a wide variety of ion channels. These toxins can be used as tools to characterize
How to easily obtain a highly purified untagged recombinant protein
Isolation of highly purified untagged proteins is crucial in today's R&D programs. Up to now, recombinant protein production approaches were often based on the use of "tags" to make protein purification
Pharma/biotech industry outsourcing trends in Biomarker studies
A recent article by E. Langer
Blood Urea Nitrogen (BUN) assays - no ammonia interference!
The determination of serum blood urea nitrogen (BUN) is currently the most widely used screening test for the evaluation of kidney function. The test is frequently run along with the serum creatinine test,
6 popular Microtubule agents used in in vitro tubulin assays
Microtubules are key components of the cytoskeletal structure of eukaryotic cells.
Cas9 mRNA optimized for genome editing
CRISPR/Cas9 is relatively simple to
Venomous toxin ion channel modulators
Ion channels are proteins with pore-forming abilities. Their selective capabilities to let ions cross the cell membrane involves them in crucial cellular functions (controlling membrance potential, the
Two new enzymes available to replace TAP
We have been closely following the interesting case of the discontinuation of tobacco acid pyrophosphatase (TAP) and the efforts of the world's RNA biologists to find a suitable alternative. Our previous