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Recent developments in 3D cell culture hydrogel
Due to their ability to simulate the characteristics of most soft tissues and natural scaffolds, hydrogels are now an attractive material for developing synthetic Extracellular Matrix (ECM) analogs.
ORF expressing Lentiviral system
ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell
Multiplex Protein Assays: which one fits you best?
Multiplex protein assays allow multiple targets quantification in a single sample, a bit like performing up to 40 different ELISAs in one go. Outsourcing Multiplex Protein Quantification projects
Antibody Purification: Tips and Tricks
For all species, classes or size (FL vs. fragments)
Whatever the type of antibody production (hybridoma or recombinant protein production
Primary cells: ready-to-use, QC tested media
In a previous post, I introduced a complete panel of ready-to-use specialized media for smooth muscle primary cells culture. Produced by Cell Applications, a GMP certified
Want to know more about your Biologics?
In new drug development pipelines, there is an increasing trend to switch from "small" to "large" molecules (also known as biologics). While the FDA guidance was quite specific with their recommendations
Accelerate your Biomarker discovery with High Density arrays
While Raybiotech recently released their 2000 target antibody array, a publication from "The Scientist" highlighted the benefit of these High-density multiplexed technology
Fully licensed SLC cell lines for FDA and EMA recommended transporters
In a previous post, we introduced our Ready-to-use cellular models for SLC assays by GenoMembrane.
These cell lines transiently express the current FDA and EMA recommended SLC
Gene knock-out in hard-to-transfect cells
Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target
New - you know CRISPR, now it's Prime Editing
This month, a new leap forward in gene editing has been published in Nature under the title 'Search-and-replace genome editing without double-strand breaks or donor DNA'. DOI: 10.1038/s41586-019-1711-4.
How can TR-FRET facilitate your cell signalling studies?
Time-Resolved Förster Resonance Energy Transfer (TR-FRET) is a robust and homogeneous fluorescence detection technology and a real alternative to ELISA to perform Cell Signalling studies.
ChIP for sensitive & fast genomic mapping
Chromatin profiling assays enable the mapping of protein-DNA interactions that influence chromatin modeling and gene expression.
Over the years, Chromatin ImmunoPrecipitation (ChIP) assays became