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How to measure Calcium in cell based assays?
In Pharma screening departments there is a strong need for Ca2+ measurement methods.
Why is this?
Immunotherapy Screening - B7-1 / CD28 and B7-1 / CTLA4 Pathways in Drug Discovery
The treatment of diseases by inducing, enhancing, or surpressing an immune response is referred to as Immunotherapy. T-cell activation and inactivation requires the coordination of various co-inhibitory
Immunotherapy Screening - A focus on PD-1/PD-L1/PD-L2 Pathway in drug discovery
The treatment of diseases by inducing, enhancing, or surpressing an immune response is referred to as Immunotherapy. T-cell activation and inactivation requires the coordination of various co-inhibitory
Specific cytokines & growth factors in... apoptosis!
Apoptosis is the process of programmed cell death. Upon induction of apoptotic processes, cell undergo characteristic morphological changes (blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation,
Pooling samples for proteomics - biomarker profiling case studies
To pool or not to pool biological samples? This question might pop up in the mind of anyone designing biomarker discovery approaches!
Much debate has been raised on biomarker discovery from clinical
5 new research tools to perform chromatin and epigenetic analysis
Nucleosomes are core particles involved in DNA packaging in eukaryotic cells. They affect gene expression levels by regulating access of DNA to transcription factors and DNA binding proteins. Nucleosome
Why multiplex histone binding arrays?
In the summer of 2013, my colleague Frederic Dubor, who is in charge of our services lab, mentioned to me that he had just been asked by 3 clients within a very short time-frame, whether our laboratory
New hope for Ras inhibitors
Ras belongs to the family of small G-proteins and plays an important role in several signal transduction pathways involved in normal cell growth and differentiation. Over 30 years ago three isoforms of
Immunotherapy - BLTA:HVEM, CD47:SIRPα in drug discovery
The treatment of diseases by inducing, enhancing, or surpressing an immune response is referred to as Immunotherapy. T-cell activation and inactivation requires the coordination of various co-inhibitory
Adipokines in obesity and glucose homeostasis
Obesity is the abnormal increase in adipose tissue mass. This phenomenon increases the likelyhood of a number of diseases such as heart disease, high blood pressure, type 2 diabetes, obstructive sleep
Immunogenicity in biotherapeutics and biosimilars
Following on from my previous post on biosimilars and biotherapeutics (or biologics), let's now take a look at some of the specific
Site-specific modified-histone substrates
Recombinant Histone proteins are popular research solutions in Drug discovery and Epigenetics studies. They can be used as in vitro positive controls in the analysis of post-translational Histone