Page 36 - ADME/Tox

Gene expression is regulated by different mechanisms. One of them is the binding of

Mechanisms leading to cellulite formation is complex. It involves lipid regulatory pathways and proinflammatory cross-talk that represent promising molecular targets in cosmetology. This post introduces

Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.

This technology produces first a targeted break in genomic DNA, which can then be exploited

Depending on the phase where your biomarker-related research program is,

Today, following our series on Tumour Microenvironment (TME), and leading on from three recent publications, let's discuss Cancer Stem Cells (CSCs).

CSCs are part of the TME, as

Hyaluronic Acid (HA) is important in many biological processes such as wound repair, tissue hydration and inflammation. HA is also a potential biomarker for diseases such as osteoarthritis, liver cirrhosis

Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol

Glycocalyx, literally meaning 'sugar coat', is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells that consists of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans.

A series of publications (1, 2) in the past months has raised the never-ending debate on commercial antibodies and their validity in a given experiment. At tebu-bio, having sold antibodies for more than

Recently I reported about methods to measure:

• Collagen - Sircol for soluble and insoluble collagen assays
• Glycosaminoglycans - How to measure Glycosaminoglycans

Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such