Page 21 - Content
How do you choose the best ELISA test for your project ?
There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let's take a look at the key points to keep in mind.
6 ELISA test formats for protein target and biomarker discoveries
Having access to highly reproducible and fully validated ELISA tests is crucial in today's Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay
Immunotherapy research: Easily build your own reporter cell line
As discussed previously in our recent Drug Discovery post, Immunotherapy is one of the central research fields for various disease treatments and also certain cancers. A major regulator
How can you improve your reporter gene assays?
Gene reporter assays are widely used in research and Drug Discovery. The development of such cell lines is often time-consuming and costly, sometimes upwards of 5-20k € per cell line.
Hit-and-run cell-based therapy research with Trilink mRNA
This summer, Moffett et al. published in Nature Communications (Nature Communications - doi:10.1038/s41467-017-00505-8
ELISA test to confirm elimination of Host Cell Proteins (HCP) from bioproductions
Expression systems - removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and
Tips for primary cell culture
Functionality and viability of primary cells can be impaired by incorrect thawing procedures, storage or culture conditions. It's generally admitted that applying the same protocols as
Biomolecule conjugation - how to biotinylate with reproducible results?
Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low
Simple and effective CRISPR CAS9 gene editing for primary cells
Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications
A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA