Page 36 - Metabolism
Click Chemistry: What it means for biologists
A few months ago I read a very nice blog post from our friends at TriLink Biotechnologies giving the chemist's perspective on the excitement surrounding "Click Chemistry" and how it can be used to make
Discovery of new biomarkers... 3 tips regarding controls
One of the recurrent questions that we get at the Biomarkers team at tebu-bio is on what controls should be included in a given experiment. Either if the experiment is done by researchers in their lab,
Focus on Actin staining and visualization
Actin serves as one of the major cytoskeleton structures. It is a crucial component involved in a plethora of processes in cell biology: stabilizing the cell shape, cell movements (e.g. cell migration)
Keep cool... corticosterone and stress
Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.
Ready-to-Use ELISAs to study Transcription Factors DNA binding
Gene expression is regulated by different mechanisms. One of them is the binding of Transcription
9 pathway-specific screening assays in Immunotherapy
Anti cellulite compound evaluation with in vitro adipocyte-assays
Mechanisms leading to cellulite formation is complex. It involves lipid regulatory pathways and proinflammatory cross-talk that represent promising molecular targets in cosmetology. This post introduces
CRISPR genome editing: which cell line to choose?
Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.
This technology produces first a targeted break in genomic DNA, which can then be exploited to produce
More data with chemiluminescence imaging platform
Depending on the phase where your biomarker-related research program is, imaging of protein or antibody arrays has different requirements. For R&D purposes
Cancer stem cells - friend or foe in the fight against cancer?
Today, following our series on Tumour Microenvironment (TME), and leading on from three recent publications, let's discuss Cancer Stem Cells (CSCs).
CSCs are part of the TME, as they reside in
From osteoarthritis to bladder cancer... hyaluronic acid is not just cosmetics!
Hyaluronic Acid (HA) is important in many biological processes such as wound repair, tissue hydration and inflammation. HA is also a potential biomarker for diseases such as osteoarthritis, liver cirrhosis
Kit-Free Site Directed Mutagenesis Protocol
Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol