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Singlet Oxygen (1O2) fluorescent detection in living cells was limited by the lack of availability of cell membrane permeable dyes. The silicon-based far-red fluorescent probe (Si-DMA) has been especially

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated proteins concentrated in lipid raft.

Lipid raft is a highly unique microdomain on the lipid bilayer which

In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!

…or how to make your protein attractive

Biomagnetic separation is a powerful lab tool in protein biochemistry which can be used in a wide range of applications including analysis tools

Small molecules known to modulate biological activies of the K-Ras oncogene are predominantly of synthetic origin (ex. Salirasib). Nevetheless, various publications

Immune checkpoint molecules play an important role in T cell functionality after TCR/MHC signaling. In fact, blockade of two B7/CD28 family checkpoint molecules, namely CTLA-4 and PD-1, have already demonstrated

Polyethylene Glycols (aka. PEGs) are biologically inert, non-immunogenic, and hydrophilic molecules. These properties make them very attractive for research and drug discovery applications (ex. Antibody-Drug

The unprecedented increase in antibiotic-resistant pathogens

Over the past months, we have released a number of posts introducing inhibitor screening assays intended to investigate immuno checkpoint

Reactive Oxygen Species (ROS) are reactive molecules and free radicals derived from molecular oxygen involved in cellular homeostasis. An excess of ROS production (e.g. exposure to environmental stress

In this post, I'd like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation,

Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of 5 optimized