Scientific Library
Article
Ready-to-Use ELISAs to study Transcription Factors DNA binding
July 06, 2015
TFACT™ DNA-BINDING ELISA KITS » OCT2 TFACT™ DNA-BINDING ELISA by Assay Biotechnology. Source: tebu-bio.
Gene expression is regulated by different mechanisms. One of them is the binding of
Application Note
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
April 17, 2018
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily