Lab Tools

Please note this product may be subject to fees, we invite you to contact your local office. The Modified RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables the extraction of cytoplasmic and nuclear proteins in mild denaturing conditions. This is an SDS-free buffer optimized and formulated for proteins that might be sensitive to some components of the standard RIPA Buffer. This buffer is compatible with many applications, including ELISA-based assays.

Please note this product may be subject to fees, we invite you to contact your local office. The RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables extraction of cytoplasmic, membrane and nuclear proteins under denaturing conditions.

Please note this product may be subject to fees, we invite you to contact your local office. This kit contains four cell lysis buffers with varying detergent strengths (from mild to strong). This kit includes 20 ml of NP-40 Cell Lysis Buffer, Modified NP-40 Cell Lysis Buffer, Modified RIPA Lysis Buffer and RIPA Lysis Buffer. This kit is intended for optimization of mammalian cell lysis conditions that best suit the assay and protein of interest.

Calcein AM solution is used for live cell staining. Calcein AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any cellular functions such as proliferation or chemotaxis of lymophocyte. The excitation and emission wavelengths of calcein are 490 nm and 515 nm, respectively.

CytoRed solution is used for live cell staining. CytoRed is cell membrane permeable and accumulates inside of viable cells as resorufin. CytoRed has a much wider spectrum than BCECF or Calcein, so filters for fluorescein and rhodamine can also be used. The excitation and emission wavelengths of resorufin are 560 nm and 590 nm, respectively.

DAPI solution is used for dead cell staining. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

PI solution is used for dead cell staining. Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.

Acridine orange (AO) solution is used as nuclear dye. AO forms a complex with double-stranded DNA to emit green fluorescence, AO also forms a complex with singlestranded DNA or RNA to emit red fluorescence. One molecule of AO intercalates with three base pairs of double-stranded DNA and emits green fluorescence with the maximum wavelength at 526 nm (excitation 502 nm). One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked, structure that emits red fluorescence with the maximum wavelength at 650 nm (excitation 460 nm)

Hoechst 33342 solution is used as nuclear dye. Hoechst dye is cell membrane permeable and stain DNA to emit intense blue fluorescence. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.