Cell-based Assays

Formaldehyde is a non-specific reactive compound with proteins, nucleic acids and lipids, and is highly toxic to all organisms. As one of the family members of Zinc-containing medium chain alcohol dehydrogenase (ADH), formaldehyde dehydrogenase is widely present in prokaryotes and eukaryotes. This enzyme can use NAD+ as a coenzyme to oxidize toxic formaldehyde and is a key enzyme in the formaldehyde oxidation pathway. FDH catalyzes formaldehyde and NAD+ to produce NADH, and the absorbance value at 340 nm increases. The activity of FDH can be reflected by the change of absorbance value of NADH at 340 nm.

Glutaminase (GLS), which is mainly found in higher animals, some bacteria and plant roots, catalyzes the hydrolysis of glutamine to glutamate and ammonia. It plays an important role in the regulation of nitrogen metabolism, especially the content of free ammonia and urea metabolism. CheKine™ Micro Glutaminase (GLS) Activity Assay Kit can detect biological samples such as animal and plant tissues, bacteria and cells, serum or plasma. In this kit, GLS catalyzes the hydrolysis of glutamine to L-glutamate and ammonia, and its enzymatic activity can be calculated by detecting the rate of ammonia increase using Nessler's reagent.

Glutamate Decarboxylase (GAD) is the rate-limiting enzyme that converts glutamate into the inhibitory neurotransmitter γ-aminobutyric acid (GABA), a potent inhibitory neurotransmitter in the central nervous system, It can reduce blood pressure, promote brain vitality, nourish nerve cells, maintain neurostability, promote growth hormone secretion, protect liver and benefit kidney, and so on. At present, it has been widely used in medicine and health food. CheKine™ Micro Glutamate Decarboxylase (GAD) Activity Assay Kit can be used to detect biological samples such as animal tissue, serum or plasma. In this kit, GAD catalysed glutamate to produce GABA, and the berthelot reaction was used to measure GABA content and thus GAD activity.

γ-Aminobutyric Acid (GABA) is a non-proteinogenic amino acid consisting of four carbon atoms, which is widely present in the organisms of plants, animals, and microorganisms. Within plants, GABA is primarily generated through decarboxylation of L-glutamate catalyzed by glutamate decarboxylase. In the mammalian brain, GABA serves as an efficacious inhibitory neurotransmitter, exhibiting functions such as lowering blood pressure, enhancing brain vitality, nourishing nerve cells, maintaining neural stability, promoting growth hormone secretion, and benefiting liver and kidney health. It has already found extensive applications in both pharmaceuticals and health food products.Principle of Determination: GABA reacts with phenol and sodium hypochlorite, giving rise to a blue-green product that exhibits a maximum absorbance at 640 nm.