Results for Cell-based Assays ( 910 )
Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK001, the actin binding protein spin-down assay kit provides G- or F-actin (rabbit skeletal muscle origin) plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. Actin binding occurs when there is an affinity for any site of actin. F-actin binding can
Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK003, The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be used to follow polymerization over time. Also, by us
BK004P, The tubulin polymerization assay is based on an adaptation of the original method of Shelanski et al. and Lee et al. which demonstrated that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The resulting polymerization curve is representative of the three phases of microtubule polymerization, namely nucleation, growth, and steady state equilibrium. The tubulin used in this assay (Cat. # HTS03) has been purified from porcine brain and consists of approximately 97% tubulin and 2% microtubule associated proteins (MAPs). This assay has been designed to give a standard polymerization yielding approximately 40-45% polymer mass. This allows the polymerization reaction to be highly sensitive to both polymerization enhancers (e.g. paclitaxel, MAPs) and polymerization inhibitors (e.g. nocodazole). The BK004 polymerization assay is suitable for screening large numbers of tubulin ligands and primary libraries. At 5 μM paclita
Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. The different actin isoforms can show distinct cellular and sub-cellular expression and localization. It has been demonstrated that different isoforms have subtly different biochemical properties in vitro which supports functional diversity within isotypes in vivo. Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. BK005 is a fluorescence format F-actin Visualization Biochem Kit. The kit utilizes fluorescently labeled phalloidin to selectively stain filamentous actin at nanomolar concentrations. They are the reagent of choice for F-actin staining of fixed cells for several reasons: 1. Bind in a stoic