ELISA

The RayBio® Phospho-IRS1 (Ser318) and Total IRS1 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates.

The RayBio® Phospho-IRS1 (Ser318) and Total IRS1 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates.

The RayBio® Phospho-IRS1 (Ser318) and Total IRS1 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates.

The RayBiotech Phospho-NFH Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human phosphorylated neurofilament heavy polypeptide (NFH) in lysate samples.

The RayBiotech Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 in lysate samples.

The RayBiotech Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 in lysate samples.

The RayBiotech Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 in lysate samples.

The RayBiotech Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 and HDAC1 in lysate samples.

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.