Functional Assays

The TAF1L (BD1 + BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1L binding to its substrate. The key to the assay kit is specific binding of the TAF1L bromodomains 1 and 2 (a.a. 1398-1649) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1L and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The TAF1 (BD 1 + BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1 binding to its substrate. The key to the kit is the specific binding of the tandem TAF1 bromodomains to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts

The ATAD2B Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2B binding to its substrate. The key to the kit is the specific binding of the ATAD2B bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2B and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The CREBBP Inhibitor Screening Assay Kit is designed to measure the inhibition of CREBBP binding to its substrate. The key to the CREBBP Inhibitor Screening Assay Kit is a specific binding of the CREBBP bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CREBBP and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The CECR2 Inhibitor Screening Assay Kit is designed to measure the inhibition of CECR2 binding to its substrate. The key to the CECR2 Inhibitor Screening Assay Kit is the highly specific binding of the CECR2 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CECR2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

The BRD4 (BD1 + BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD4 (BD1 + BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

The BRD4 (BD1) TR-FRET Assay Kit is designed to measure the inhibition of BRD4 (BD1) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

The BRD2 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD2 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD2, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

The BRD3 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD3 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD3, ligand, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.