Bioconjugation Kits

S-HyNic Linker (DMF Soluble), 10 mg. S-HyNic heterobifunctional crosslinker is fundamental to SoluLINK bioconjugation technology. S-HyNic reacts with primary amines on proteins (through lysine amino acids) or amino-modified oligonucleotides or surfaces, introducing a HyNic linker that forms stable covalent conjugates with biomolecules possessing 4FB linkers.

S-4FB Linker (DMF Soluble), 10 mg. S-4FB heterobifunctional crosslinker is fundamental to SoluLINK bioconjugation technology. S-4FB reacts with primary amines on proteins (through lysine amino acids) or amino-modified oligonucleotides or surfaces, introducing a 4FB linker that forms stable covalent conjugates with biomolecules possessing HyNic linkers.

S-HyNic Linker (DMF Soluble), 5 x 1 mg. S-HyNic heterobifunctional crosslinker is fundamental to SoluLINK bioconjugation technology. S-HyNic reacts with primary amines on proteins (through lysine amino acids) or amino-modified oligonucleotides or surfaces, introducing a HyNic linker that forms stable covalent conjugates with biomolecules possessing 4FB linkers.

TurboLINK Catalyst Buffer (10X), 1.5 mL, is used to catalyze SoluLINK bioconjugation reactions, greatly accelerating hydrazone formation, with the conjugation reaction typically complete in 1–2 hrs. The active ingredient, aniline, is delivered at 100 mM concentration in Conjugation Buffer. A final concentration of 5–10 mM aniline in the conjugation reaction is sufficient for catalysis. This is particularly useful for conjugation of large biomolecules (e.g., antibodies, oligonucleotides, etc.).

Modification Buffer (10X), 5 x 1.5 mL, is an amine-free, high buffering capacity solution that is optimized for the reaction of NHS esters and TFP esters with amino groups. Proteins, oligonucleotides, and other biomolecules are buffer exchanged into Modification Buffer prior to modification with S-HyNic and S-4FB to ensure efficient coupling of these linkers.

Conjugation Buffer (10X), 5 x 1.5 mL, is a high buffering capacity solution that is optimized for the conjugation reaction between HyNic and 4FB groups. Proteins, oligonucleotides, and other biomolecules are buffer exchanged into Conjugation Buffer after modification with S-HyNic and S-4FB, and prior to conjugation, to ensure efficient conjugation of these linkers.

Bilirubin Conjugate is a water soluble ditaurate derivative of bilirubin equivalent to bilirubin glucuronide derivatives. Bilirubin glucuronide derivatives are produced in vivo from the glucuronidation (addition of one or two glucuronic acids through a glycosidic bond) of bilirubin (also called indirect bilirubin) in a reaction catalyzed by the enzyme UDP Glucuronidase. The glucuronidation of bilirubin occurs in the liver to form the water soluble bilirubin mono or diglucuronide (also called bilirubin conjugate or direct bilirubin). Bilirubin conjugate is excreted from the liver in bile or is converted to urobilinogen and excreted in the urine as urobilin or in the feces as stercobilin.References1) Maghzal G. J. M.-C. Leck et al. (2009). "Limited Role for the Bilirubin-Biliverdin Redox Amplification Cycle in the Cellular Antioxidant Protection by Biliverdin Reductase." Journal of Biological Chemistry 284(43): 29251-29259.2) MacLean P. D. E. C. Drake et al. (2007). "Bilirubin as a

The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells withi

The Collagen Hybridizing Peptides are products of 3-Helix Inc.The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains both in vitro and in vivo [1 2 3]. By sharing the Gly-X-Y repeating sequence of natural collagen CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites and it is inert towards non-specific binding because of its neutral and hydrophilic nature.CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases as well as tissue remodeling during development and aging [3]. CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells withi