Single-domain antibodies: Novel Llama immunoreagents

A single-domain antibody (sdAb, also called Nanobody) is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind selectively to a specific antigen. With a molecular weight of only 12–15 kDA, single-domain antibodies are much smaller than common antibodies (150–160 kDa). They have been shown to be just as specific as a regular antibody and in some cases more robust. They are being researched for multiple pharmaceutical applications in in vivo imaging and targeted therapy. eg. the fusion of a fluorescent protein to a single-domain antibody can be used to trace targets in different compartments of living cells. They can therefore increase the possibilities of live cell microscopy and will enable novel functional studies.

As this technology was originally developed following the discovery that camelidae, including llamas, possess fully functional antibodies that consist of heavy chains only, Rockland has developed a whole range of camelid-related products to provide a full single domain antibody toolbox. This range goes from the llama serum, to control Ig isotype, and anti-VHH antibody.

Part of this product range, the rabbit anti-Llama Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for Llama immunoglobulin IgG1 and IgG2. They are useful in the detection, sorting or purification of its specified target- VHH domain or antibody, suitable for western blot, ELISA, ChIP and immunohistochemistry.

Are you interested in these antibodies? Convenient trial sizes are available for testing. If you’d like to learn more, please get in touch by leaving a comment below!


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