CRISPR-CAS9 is a powerful technology for gene editing. It allows targeted modifications into the genome of the cell lines, IPSCs and ESCs. Despite the principle being pretty simple, in practice its use requires some expertise, and it can even turn into a time consuming adventure… That’s why the best way to benefit from it is most certainly through affordable services.
Simplifying gene editing
We can distinguish 2 kinds of gene editing. Knock-out (KO) of a gene or a miRNA to abolish the corresponding function. Knock-in (KI) is the modification of the target. KI can be point mutation, gene tagging, exon swapping, SNP allele editing. Actually, gene editing can be anything we need for research.
KO cell line service
To make it as easy as possible, you just need to specify the cells and the targeted gene, and then 4 months later you’ll receive the monoclonal KO cell line with genotyping by NGS.
The service includes:
- Design and validation of reagents
- Clonal expansion
- NGS genotyping
Ask for your quote right here. It’s very affordable!
KI cell line service
It’s a little be more sophisticated and you’ll need to mention the modification. Just provide the targeted sequence and indicate with * the position of the insertion. For point mutation, please indicate the base to modify and the 20 bases in 3′ and in 5′. Then, 6 months later you’ll receive the monoclonal KI cell line with genotyping NGS.
Here again, the goal is to make gene editing simple and affordable. So don’t wait and ask for your quote here!
Our gene editing experts are at Canopy laboratories at St Louis in the USA.
2 Responses
We would like to use CRISPR-Cas9 swapping onto yeast genomic DNA, this after integration of a GFP gene (therefore to be removed then) . Do you have such experience and material to provide us 2gRNA for swapping the GFP gene surrounding sequence, as well as the Cas9 protein to be transferred to the yeast ?
Sincerely
Our CRISPR-CAS9 reagents, tool boxes and services are for Mammalian cells. A typical example is our CAS9 mRNA (https://www.tebu-bio.com/s/h7qvwq) that was codon-optimized for Mammalians. Nevertheless, our CRISPR boxes (https://www.tebu-bio.com/s/2hlku7) contain the CAS9 protein, unmodified, that should work for yeast. Concerning gRNAs, they are some kind of universal guide for CRISPR working in a lot of species including bacteria and eukaryotes. It is just a question of design specific to the project.