ATRA is a well known for its ability to regulate cell differentiation. However, retinoids are excellent chromophores and efficiently absorb light and isomerise. This is not necessarily a feature required of cell culture reagents, where consistency and reliability of a product is important for robust reproducible results.
To address this issue, Reinnervate have recently developed ec23® as a pan-RAR receptor agonist that is stable, but maintains the same biological activity as ATRA.
Robust induction of Neurogenesis by ec23®
Reinnervate has demonstrated that synthetic retinoid ec23® is a potent inducer of pluripotent stem cell differentiation. Human TERA2.cl.SP12 pluripotent stem cells exposed to either 10 μM ec23® or ATRA form populations of terminally differentiated neurons. Immunocytochemical staining for neurofilament-200 was performed on differentiated cultures and the number of positive cells quantified. Results show that ec23® is a more potent inducer of neurogenesis than ATRA. In direct comparison to ATRA, they found that synthetic retinoid ec23® induces larger numbers of neural cells with less variability and with the added advantage of compound stability.
Synthetic retinoid ec23® is also a potent inducer of neurogenesis by adult neuroprogenitor cells. Rat adult hippocampal progenitor cells (AHPCs) exposed to either 1 μM ec23® or ATRA differentiate into neural cell types. Differentiation induced by exposure to ec23® was consistent and reproducible.
Product Specifications
| Size | 10 mg (Cat. No. SR-P002-2) |
| Alternate Name | 4-(5,5,8,8-Tetramethyl-5,6,7,8-tetrahydronaphthalen-2-ylethynyl) benzoic acid |
| Chemical Formula | C23H24O2 |
| Molecular Weight | 332 |
| CAS Number | 104561-41-3 |
| Purity | Greater than 99% by HPLC analysis |
| Formulation | Off-white solid |
| Solubility | Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet. |
| Storage and Stability | Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. |
| Quality Control | The purity of ec23 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. |
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