Nucleic Acids

Total RNA was prepared from cell line WM3619. WM3619 is a metastatic human melanoma cell line that displays a mesenchymal morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3619 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3623. WM3623 is a metastatic human melanoma cell line. This cell line was established from a right lymph node metastasis site in a 48-year-old male with level V metastatic malignant melanoma. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM3623 cells produce xenograft tumors when injected into immunocompromised mice.

DNA was prepared from cell line WM3623. WM3623 is a metastatic human melanoma cell line. This cell line was established from a right lymph node metastasis site in a 48-year-old male with level V metastatic malignant melanoma. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM3623 cells produce xenograft tumors when injected into immunocompromised mice.