Buffers & Concentrates

CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer: mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells) human bone marrow derived macrophages human TIG-1 fetal lung cells human monocyte derived macrophages human LoVo colorectal cancer cells human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs) human MCF7 breast cancer cells human intestinal tissue human MDA-MB-231 bre

DMEM/F-12 Medium (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEM medium and Ham's F-12 medium, based on DMEM medium. F-12 was added to enrich the nutrient content, containing a variety of trace elements. Dulbecco Modified Eagle Medium (DMEM) is a modified Eagle Basic Medium (BME) with four times the amino acid and vitamin concentration of BME. Ham's F-12, based on Ham's F-10 medium, significantly increased the concentration of choline, inositol, putputylamine and several amino acids. DMEM/F-12 medium is widely used to support the growth of a variety of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the culture of mammalian cells with low serum content and clone density. DMEM/F12 Medium contains amino acids, vitamins, inorganic salts and other components required for