Cells Reagents

McCoy's 5A medium is a medium designed by Thomas McCoy et al in 1959, originally specifically for the culture of Novikoff Hepatoma cells. McCoy's 5A is a modified Medium 5A that contains reduced glutathione, bacterial peptone and a high concentration of glucose. McCoy's 5A is widely used in the culture of many types of primary cells, such as bone marrow, skin, gum, kidney, spleen, lung, rat embryo, omentum, etc. In addition, McCoy's 5A medium is used for tissue biopsy culture, cell line construction, and some lymphocyte and difficult-to-culture cells culture. McCoy's 5A Medium contains amino acids, vitamins, inorganic salts and other components required for multi-type cell culture, but does not contain lipids or any growth factors, so the product should be used with or without serum additives. This product is filtered with 0.22 μm filter membrane to remove bacteria, without high temperature and high pressure sterilization, less nutrient loss, ready to open the bottle; To provide a vari

T cells are important immune cells that have a variety of functions. T cells can eliminate infected or cancerous cells and direct the immune response by helping B lymphocytes eliminate invading pathogens. T cells play a critical role in destroying cancer cells by recognizing antigens presented on cancer cells. CAR (chimeric antigen receptor)-T cell therapy has proven to be effective against some types of cancer, even when other treatments are not effective. T cells in general are widely used in cancer and research of other human diseases. However, T cells require activation to become active and proliferate. In vitro activation typically involves the use of CD3 and CD28 antibodies, in multiple forms, to engage the T cell receptor and use a co-stimulatory protein, attempting to mimic the situation in vivo where T cells are activated by the MHC (major histocompatibility complex) and APC (antigen presenting cells). This process of activation and expansion is crucial to generate enough cell

T cells are important immune cells that have a variety of functions. T cells can eliminate infected or cancerous cells and direct the immune response by helping B lymphocytes eliminate invading pathogens. T cells play a critical role in destroying cancer cells by recognizing antigens presented on cancer cells. CAR (chimeric antigen receptor)-T cell therapy has proven to be effective against some types of cancer, even when other treatments are not effective. T cells in general are widely used in cancer and research of other human diseases. However, T cells require activation to become active and proliferate. In vitro activation typically involves the use of CD3 and CD28 antibodies, in multiple forms, to engage the T cell receptor and use a co-stimulatory protein, attempting to mimic the situation in vivo where T cells are activated by the MHC (major histocompatibility complex) and APC (antigen presenting cells). This process of activation and expansion is crucial to generate enough cell

NK (natural killer) cells are part of the innate immune system. They function in a histocompatibility complex-independent mode and derive from the hematopoietic lineage. In PBMCs (human peripheral blood mononuclear cells) derived from healthy individuals, 5-20% of the cells are NK cells. They are the first line of defense against cancer. Expression of marker CD56 correlates with NK cell functionality: the CD56^bright subset accounts for about 5% of the population and is less cytotoxic than the CD56^dim subset.  Cytotoxicity can happen by the release of perforin and granzyme, while activation by KARs (killer activating receptors) leads to release of Fas Ligand, TRAIL (TNF-related apoptosis-inducing ligand) and TNFα (tumor necrosis factor-alpha). In a suppressive tumor microenvironment, NK cells can become inhibited and unable to fight cancer cells. Several clinical trials have focused on using ex vivo generated NK cells alone or in combination with other approaches.