Adenovirus

Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. This pre-packaged adenovirus over-expresses Cre recombinase under CMV promoter.

This recombinant human adenovirus type 5 expresses the mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase (Mito) fusion with EGFP under the CMV promoter. The Mito sequence is fused to the 5'-end of EGFP. The Mito sequence targets the Mito-EGFP fusion protein to the host cell’s mitochondria.