Proteins & Peptides

Human Pyruvate kinase M2 (PKM2) also known as PKM1/M2, isoform a (GenBank Accession No. NM_002654) full length with N-terminal His-tag, MW = 58.9 kDa, expressed in an E. coli expression system.

Mouse DNA methyltransferase 3β??? (DNMT3B), GenBank Accession No. NM_001003961, a.a. 2-859 (end), with N-terminal His-tag and C-terminal FLAG-tag, MW = 99 kDa, expressed in Sf9 cells via a Baculovirus expression system.

Human COX2, GenBank Accession No. NM_000963, a.a. 1-604 (end), with C-terminal FLAG-His-tag, MW=71 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Recombinant human IL-6 is a glycosylated disulfide-linked homodimeric protein consisting of 184 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in E. coli.

Recombinant TNF-alpha is a disulfide-linked monomer protein, consisting of 158 amino acids and migrates as an approximately 17 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha chain was expressed in E. coli.

Lipoxygenase Inhibitor Screening Assay Kit is a measurement of detecting hydeoperoxides produced in the lipoxygenation reaction.

CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [1]. CUT&Tag offers significant improvements in signal to noise at reduced cell inputs and sequencing depth compared to ChIP-seq. As a fusion of Proteins A and G to a highly active E. coli transposase mutant (Tn5), CUTANA pAG-Tn5 is compatible with target antibodies from a broad spectrum of host species. It also comes without an epitope tag, making it suitable for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This product is highly purified to remove contaminating E. coli DNA, which can be tagmented during storage and complicate analysis at low cell numbers. For superior normalization as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. The Tn5 comes charged with mosaic adapters and is ready to be used in CUT&Tag.