RNA

Total RNA was prepared from cell line WM983C. WM983C is a metastatic human melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type PTEN, N-RAS, c-KIT, and CDK4 genes. WM983 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM989. WM989 is a melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM989 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM989. WM989 is a melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM989 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM989. WM989 is a melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type N-RAS, c-KIT, and CDK4 genes. WM989 cells produce xenograft tumors when injected into immunocompromised mice.

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. This mRNA is capped using CleanCap® Reagent M6, TriLink’s patented co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified with N1-methylpseudouridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA.

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. This mRNA is capped using CleanCap® Reagent M6, TriLink’s patented co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified with N1-methylpseudouridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA.

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. This mRNA is capped using CleanCap® Reagent M6, TriLink’s patented co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified with N1-methylpseudouridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA.

The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin. This mRNA is capped using CleanCap® Reagent M6, TriLink’s patented co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified with N1-methylpseudouridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA.