Nucleic Acids

DNA was prepared from cell line WM1727. WM1727A is a metastatic human melanoma cell line that displays pigmented melanocytic morphology and float easily in culture medium. WM1727A cells produce xenograft tumors when injected into immunocompromised mice. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for N-RAS, c-KIT, and CDK4.

Total RNA was prepared from cell line WM1727. WM1727A is a metastatic human melanoma cell line that displays pigmented melanocytic morphology and float easily in culture medium. WM1727A cells produce xenograft tumors when injected into immunocompromised mice. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for N-RAS, c-KIT, and CDK4.

Total RNA was prepared from cell line WM1727. WM1727A is a metastatic human melanoma cell line that displays pigmented melanocytic morphology and float easily in culture medium. WM1727A cells produce xenograft tumors when injected into immunocompromised mice. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for N-RAS, c-KIT, and CDK4.

Total RNA was prepared from cell line WM1727. WM1727A is a metastatic human melanoma cell line that displays pigmented melanocytic morphology and float easily in culture medium. WM1727A cells produce xenograft tumors when injected into immunocompromised mice. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for N-RAS, c-KIT, and CDK4.

DNA was prepared from cell line WM1789. WM1789 is a human melanoma cell line originally isolated from a human primary radial growth phase melanoma. WM1789 cells produce xenograft tumors when injected into immunocompromised mice. This line carries the K601E and S363F mutation in BRAF, and is hemizygous deleted for PTEN.

DNA was prepared from cell line WM1789. WM1789 is a human melanoma cell line originally isolated from a human primary radial growth phase melanoma. WM1789 cells produce xenograft tumors when injected into immunocompromised mice. This line carries the K601E and S363F mutation in BRAF, and is hemizygous deleted for PTEN.

DNA was prepared from cell line WM1789. WM1789 is a human melanoma cell line originally isolated from a human primary radial growth phase melanoma. WM1789 cells produce xenograft tumors when injected into immunocompromised mice. This line carries the K601E and S363F mutation in BRAF, and is hemizygous deleted for PTEN.

Total RNA was prepared from cell line WM1789. WM1789 is a human melanoma cell line originally isolated from a human primary radial growth phase melanoma. WM1789 cells produce xenograft tumors when injected into immunocompromised mice. This line carries the K601E and S363F mutation in BRAF, and is hemizygous deleted for PTEN.

Total RNA was prepared from cell line WM1789. WM1789 is a human melanoma cell line originally isolated from a human primary radial growth phase melanoma. WM1789 cells produce xenograft tumors when injected into immunocompromised mice. This line carries the K601E and S363F mutation in BRAF, and is hemizygous deleted for PTEN.