ELISA Reagents

The one component 3,3?,5,5?-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths. Benefits of the BioFX� family of substrates include: Prolonged stability. Increased sensitivity. Neglibible backgrounds. Ease of Manufacturing.

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths. Benefits of the BioFX™ family of substrates include: Prolonged stability Increased sensitivity Negligible backgrounds Ease of manufacturing

The one component 3,3?,5,5?-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths. Benefits of the BioFX� family of substrates include: Prolonged stability. Increased sensitivity. Neglibible backgrounds. Ease of Manufacturing.

ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a colorimetric substrate suitable for peroxidase (HRP) enzyme labeling, producing a green color upon oxidation that is measurable at 405-410 nm. ABTS is preferred for applications requiring a soluble end product and is compatible with both plate readers and spectrophotometers.

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change is quantifiable at 450 nm. TMB's high sensitivity makes it ideal for detecting low concentrations of analytes.

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change is quantifiable at 450 nm. TMB's high sensitivity makes it ideal for detecting low concentrations of analytes.

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change is quantifiable at 450 nm. TMB's high sensitivity makes it ideal for detecting low concentrations of analytes.

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change is quantifiable at 450 nm. TMB's high sensitivity makes it ideal for detecting low concentrations of analytes.

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid.