Cell Line

Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office. This product is provided at this price to academic organisations (not for profit).  In vitro, only a small number of primary oligodendrocytes express GFAP and GFAP+ astocytes rarely express oligodendrocytic markers. MO3.13, an immortal human-human hybrid cell line that express phenotypic characteristics of primary oligodendrocytes, was created by fusing a 6-thioguanine-resistant mutant of the human rhabdomyosarcoma RD with adult human oligodendrocytes by a lectin-enhanced polyethylene glycol procedure. In contrast to the tumor parent, M03.13 expressed surface immunoreactivity for galactosyl cerebroside(GS) and intracellular immunoreactivity for myelin basic protein (MBP), proteolipid protein (PLP), and glial fibrillary acidic protein (GFAP).

Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office. This product is provided at this price to private organisations.

Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office. This product is provided at this price to academic organisations (not for profit).  The Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) was prepared from cerebral microvessel endothelial cells (CECs) by transduction with lentiviral vectors carrying the SV40 T antigen and human telomerase reverse transcriptase (hTERT). This cell line shows a spindle-shaped, elongated morphology similar to primary cultures of brain endothelial cells and also exhibits contact inhibition at confluence when cultured on collagen type I or IV. In addition, this line expresses a variety of brain endothelial markers, adherence junction (AJ) and tight junction (TJ) proteins as well as functional ABC transporters typical to brain epithelium. hCMEC/D3 represents a stable, easily grown blood brain barrier (BBB) model cell line.

CF-1 Mouse Embryonic Fibroblast (MEF) feeder cells, Mitomycin-C treated. Mitotically inactivated fibroblasts should be used as a support layer for embryonic stem (ES) cells and induced pluripotent stem (iPS) cells.

The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964). Please note this product may be subject to fees, we invite you to contact your local office. Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office.

Recombinant CHO-K1 cell constitutively expressing full-length human CD137L (NP_003802.1). Surface expression is confirmed by flow cytometry. Please note this product may be subject to fees, we invite you to contact your local office. Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office.

Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294. Please note this product may be subject to fees, we invite you to contact your local office. Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office.

Recombinant HEK293 cell line expressing full length human CD155, also known as poliovirus receptor (PVR) or nectin-like molecule-5 (NECL-5). Expression is confirmed by Western Blot. Please note this product may be subject to fees, we invite you to contact your local office. Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office.

Recombinant CHO-K1 cells constitutively expressing human CD155 (Poliovirus receptor, Nectin-like protein 5, GenBank accession #NM_006505) and an engineered T cell receptor (TCR) activator. Please note this product may be subject to fees, we invite you to contact your local office. Please note that an MTA needs to be signed prior to purchase of this product, we kindly request that you contact your local office.