Challenged by low cell transfection of your mRNA using liposome carriers?
Maybe it’s due to their intrinsic instability: as they are only composed of fluid lipids, they tend to fuse with each other. This lack of rigidity generally leads to mRNA loss prior to transfection, and thus to low cell transfection.
Our solution for you to overcome this problem, is the new i-LipoP® solution (Adjuvatis).
What are the added values of the i-LipoP® mRNA delivery tool?
This innovative and easy-to-use mRNA delivery tool is based on 220nm lipoparticles consisting of polylactic acid nanoparticles coated with a lipid membrane.
- Solid core – acts as a cytoskeleton to confer mechanical stability when taken up into cells by endocytosis
- Large flexibility for experiment planning – up to 48 hours can be allowed between formulation and transfection ! It’s easier for you to anticipate and plan technical steps of your project
- Much higher transfection capacity for hard-to-transfect cells – i-LipoP® can be used to unlock transfection issues, exhibiting high delivery efficiency in cytoplasm while limiting toxicity
- Wide range of sizes for mRNA transfection – from 300 bp to 3 kb mRNA at least
To the right, you can see results of higher mRNA transfection efficiency obtained using i-LipoP® in 2 hard-to-transfect cell lines, namely Epithelioma papulosum cyprini and CHSE-214 (Chinook Oncorhynchus tshawytscha specie), compared to Trans IT® and Lipofectamine® transfection kits.
Moreover, the particulate layer-by-layer (pLbL) strategy of i-LipoP® uses electrostatic interactions to successively adsorb mRNA and a cell-penetrating peptide on the surface of lipoparticles to facilitate endosomal escape, enhancing the transfection capacity of the carrier.
References
Camille Ayad et al (2021). LipoParticles: Lipid-Coated PLA Nanoparticles Enhanced InVitro mRNA Transfection Compared to Liposomes. Pharmaceutics 13, 377