Beads & Microspheres

Tyrosine phosphorylation, a reversible process, is a frequent post translational modification of proteins and is crucial in mediating signal transduction in eukaryotic cells after exposure to cytokines and growth factors. As part of the Signal Seeker product line, phosphotyrosine affinity beads have been optimized to detect endogenous levels of phosphotyrosine modified proteins, which often represent <1% of the target protein. Phosphotyrosine affinity beads comprise an anti phosphotyrosine antibody (Clone 27B10) that has been chemically conjugated to Protein G beads. Validation studies have shown that these beads can immunoprecipitate a wide range of phosphotyrosine proteins without detectable leaching of either heavy or light chains in an IP assay. APY03 Beads have been shown to enrich a wide range of tyrosine phosphorylated proteins in HeLa cells treated with pervanadate and A431 cells treated with EGF and to efficiently enrich specific phosphotyrosine proteins from crude cell lysat

SUMOylation is a dynamic and reversible process that plays pivotal roles in transcriptional regulation, chromatin remodeling, response to stress and regulation of mitosis. Signal Seeker SUMOylation 1 products provide a simple and effective way to determine if your target protein is modified and regulated by this PTM. As part of the Signal Seeker product line, SUMOylation 1 affinity beads have been optimized in order to detect endogenous levels of SUMO1 modified proteins, which often represent <1% of the target protein. SUMOylation 1 affinity beads comprise an anti SUMO1 antibody (Clone 7A1A2) that has been chemically conjugated to Protein G beads. ASM11 beads enrich a more complete profile of SUMO1 modified proteins from HAP1 wildtype cells while also minimizing non specific interactions versus other SUMO1 affinity reagents. ASM11 affinity beads are supplied as a lyophilized white powder and are valied for IP application.

SUMOylation is a dynamic and reversible process that plays pivotal roles in transcriptional regulation, chromatin remodeling, response to stress and regulation of mitosis. Signal Seeker SUMOylation 2/3 products provide a simple and effective way to determine if your target protein is modified and regulated by this PTM. As part of the Signal Seeker product line, SUMOylation 2/3 affinity beads,ASM24 beads, have been optimized in order to detect endogenous levels of SUMO 2/3 modified proteins, which often represent <1% of the target protein. SUMOylation 2/3 affinity beads comprise an anti SUMO 2/3 antibody (Clone 11G2) that has been chemically conjugated to Protein G beads. The linear epitope has not been identified and seems recognize a conformational epitope. ASM24 Beads are supplied as a lyophilized powder and are validated for IP applications.

As part of the Signal Seeker product line, IgG control beads (CIG01) have been developed as highly specific negative control beads for Cytoskeleton's mouse, monoclonal antibody based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition.Mouse IgG IP Control Beads provide an ideal negative control and should be included in an IP experiment to control for non specific binding in any antibody based affinity immunoprecipitation reaction. 

As part of the Signal Seeker product line, acetyl lysine control beads (CIG02) have been developed as highly specific negative control beads for Cytoskeleton's acetyl lysine antibody based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition.Mouse IgG IP Control Beads provide an ideal negative control and should be included in an IP experiment to control for non specific binding in any antibody based affinity immune precipitation reaction.

As part of the Signal Seeker product line, SUMO1 IP control beads (CIG03) have been developed as highly specific negative control beads for Cytoskeleton's SUMOylation 1 antibody based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. Mouse IgG IP Control Beads provide an ideal negative control and should be included in an IP experiment to control for non specific binding in any antibody based affinity immuneprecipitation reaction.

Protein ubiquitination is a regulatory mechanism that controls protein:protein interactions, protein degradation, and spatial localization, which is mediated by chain specific poly ubiquitination and/or mono ubiquitination. Signal Seeker ubiquitination tools have the unqiue ability to capture both mono and poly ubiqutination for any target protein. As part of the Signal Seeker product line, Ubiquitin affinity beads, UBA01 beads, have been optimized in order to detect endogenous levels of mono and poly ubiqutinated proteins, which often represent <1% of the target protein. UBA01 Beads can enrich all ubiquitinated species with a high affinity, including mono , multi and polyubiquitin chains. The affinity moeities have been cross linked to beads for low leaching and cleaner detection of the protein of interest.