Click Chemistry

SiR-NHS ester is the active ester form of SiR. SiR-NHS ester reacts with amines to form stable covalent amide bond

SiR-NHS ester is the active ester form of SiR. SiR-NHS ester reacts with amines to form stable covalent amide bond

SiR-COOH ester is the carboxylic acid form of SiR. SiR-COOH can be activated and coupled to ligands or linkers for the preparation of custom conjugate.

SiR-COOH ester is the carboxylic acid form of SiR. SiR-COOH can be activated and coupled to ligands or linkers for the preparation of custom conjugate.

SiR-tetrazine is a clickable derivative of SiR. This probe reacts with dienophiles such as trans-cyclooctene (TCO) or bicyclononyne (BCN) derivatives, enabling the preparation of custom SiR probes and conjugates. SiR-tetrazine is particularly useful in combination with unnatural amino acid (UAA) incorporation to prepare site selective protein conjugates. SiR-tetrazine is cell permeable and fluorogenic, it can be used to stain live cells as well as fixed ones. SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

SiR-azide is a clickable derivative of SiR. It reacts with alkynes and allows the preparation of custom SiR-conjugates

SiR-alkyne is a clickable derivative of SiR. It reacts with azides and allows the preparation of custom SiR-conjugates.

SiR-maleimide is the 2-maleimidoethyl derivative of SiR. It reacts with thiols and allows the preparation of custom SiR-conjugates

SiR-BCN is a clickable derivative of SiR. It reacts with tetrazine or azide derivatives and allows the preparation of custom SiR-conjugates