Cell Line

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell constitutively expressing full length human SIRPα (#NM_080792). Surface expression is confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell transfected with tetracycline repressor gene. This cell line is validated for response to the stimulation of doxycycline.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line over-expressing full length human CD47 (NM_198793.2). Surface expression is confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant Jurkat T cell expressing a firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIa, Histidine variant.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human LAG-3 (lymphocyte-activation gene 3, CD223, GenBank Accession # NM_002286).

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein (also known as B4 or CVID3, Genbank #NM_001770), human BCMA protein (B-Cell Maturation Antigen or CD269, GenBank accession #NM_001192) and the firefly luciferase. Surface expression of CD19 and BCMA were confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing MDA-MB-231 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.