Delivery Tools

The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the TWO-Step Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency.

Broadly Acting Transfection Reagent for mRNA Delivery Provides effective delivery into attachment dependent and suspension cells Optimized for delivery of CRISPR mediators: Cas9 (RNP) complexes as well as pDNA / mRNA complexes for CRISPR

This product is designed for mRNA delivery of both attachment-dependent and attachment-independent (suspension growing) cells. mRNA-Fect has also been tested and found effective for plasmid DNA and siRNA delivery in certain cell types. We recommend the users to test the efficacy of the reagent in their particular cell type and choose the right formulation for long-term use.

By utilizing our innovative and proprietary lipid conjugation technology, LipoD293™ (Ver. II) is specially designed and formulated by adding proprietary enhancers for transfecting HEK293 cells and other mammalian cells (Figure 1). As a 2nd generation liposome based DNA transfection reagent, LipoD293™ (Ver. II) offers extremely high transfection efficiencies for HEK293 related cells as well as many mammalian cells with less cytotoxicity. LipoD293™ reagent (Ver. II), upgraded from its previous version with refined chemistry, is 3~4 times more efficient in gene delivery. LipoD293™ reagent (Ver. II), 1.0 ml, is sufficient for ~666 transfections in 24 well plates or ~333 transfections in 6 well plates.

Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. The PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.