Results for Assays & Kits ( 14061 )
- From: £232.00
Find our optimized NADPH regeneration system and catabolism buffer to pair with your in vitro assays using recombinant enzymes and subcellular fractions. RapidStart™ is a versatile system that supports extended metabolism of test articles by NADPH-dependent drug-metabolizing enzymes in subcellular fractions for in vitro drug metabolism assays. The system is based on Glucose-6-Phosphate DeHydrogenase (G6PDH) and Glucose-6-Phosphate (G6P), with reduction of NADP+ to NADPH. NADPH is a cofactor required to support reactions by drug-metabolizing enzymes such as cytochrome P450 (CYP) and flavin-containing monooxygenases (FMO). Assays using microsomes, S9 and recombinant CYPs (Bactosomes) can be supported with RapidStart™ to support the long-term metabolism of test articles by these enzymes by ensuring that NADPH is not the limiting reagent.
- From: £279.00
The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time • High-throughput: simple homog
- From: £726.00
The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
- From: £992.00
The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.