Labelling

Annexin V specifically binds to phosphatidylserine in the presence of protein-dependent Ca ion. By using fluorescent-labeled Annexin V, the apoptotic cells can be identified by flow cytometry or fluorescence microscopy.

Bathocuproinedisulfonic acid is a water-soluble analog of bathocuproine that emits fluorescence under UV light.

BCECF is the most widely used intracellular pH probe. BCECF is highly water-soluble because it has 4 to 5 negative charges at neutral pH; it becomes difficult to pass through the cell membrane after loading. Its pKa value, 6.97, is higher than that of carboxyfluorescein. BCECF has an isosbestic point at 439 nm in the excitation spectra, so it can be used in ratiometry, similar to Fura 2.

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent