Assays & Kits

Nitrite reductase (NiR) is a crucial enzyme in the reduction process of nitrite nitrogen and plays a vital role in the nitrogen cycle of nature. It is widely distributed in microorganisms and plants, capable of catalyzing the reduction of nitrite, thereby decreasing the accumulation of nitrite nitrogen in the environment and alleviating its toxic effects on the growth and development of organisms. CheKine™ Micro Nitrite Reductase (NiR) Activity Assay Kit is designed to quantify nitrite reductase activity in plant tissues, bacterial, and fungal samples. It operates on the principle that nitrite reductase catalyzes the reduction of NO2- to NO, thereby reducing the amount of NO2- available in the sample to participate in diazotization reactions that form a purple-colored compound. The change in absorbance at 540 nm reflects the activity of NiR in the sample.