Sub Cellular Fraction

ZenBio human pre-adipocytes (adipose derived adult stem cells) and placental mesenchymal stem cells (MSC) are characterized by their self-renewing capacity and ability to differentiate into chondrocytes, adipocytes, and osteocytes. This makes them attractive starting materials for tissue engineering and regenerative medicine applications. Quality Control: All cells have been screened negative for HIV-1, HIV-2, Hep-B, Hep-C and Syphilis. All isolated and concentrated exosomes undergo includes Particle mean diameter, Protein concentration, RNA concentration and Concentration of particles/ml.

ZenBio human pre-adipocytes (adipose derived adult stem cells) and placental mesenchymal stem cells (MSC) are characterized by their self-renewing capacity and ability to differentiate into chondrocytes, adipocytes, and osteocytes. This makes them attractive starting materials for tissue engineering and regenerative medicine applications. Since few transplanted cells persist in vivo, the beneficial effects of cell therapy may lie in the secreted factors being the active component of this treatment. A key part of paracrine secretion is Exosomes, which are membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane.

Human liver microsomes contain a wide variety of drug metabolizing enzymes and are a recommended test system for assays including metabolite identification (MetID), metabolic stability, enzyme inhibition, reaction phenotyping, and other in vitro drug metabolism and pharmacokinetics (DMPK) studies. We offer pooled microsomes with representative averages of extensive and wide-ranging inter-individual differences in enzyme activity found in the general North America/USA population, as well as preparations from individual donors that have been genotyped for the presence of a specific single nucleotide polymorphism (SNP). Genotyped human liver microsomes can be used to investigate the influence of allelic variance on the safety and efficacy of new molecular entities. Please, contact your local Tebubio office for lot availability, specific criteria and to receive the lot-specific datasheet of your product.

Using intestinal subcellular fractions as a test system is highly recommended due to the importance of intestinal enzymes in the first-pass metabolism of many orally ingested xenobiotics, including cytochrome P450 (CYP), UDP-glucuronosyltransferase (UGT), and esterase enzymes. Consequences of intestinal biotransformation can be very significant, including toxification by bioactivation and detoxification by aiding in excretion.We offer pooled intestinal subcellular fractions demonstrating high enzyme activity levels. Options include microsomes, S9, and cytosol, from matching large donor pools to minimize variability. Intestinal microsomes are supplied in 250 mM sucrose buffer. S9 and cytosolic fractions are supplied in a homogenization buffer with or without Phenylmethylsulfonyl flouride (PMSF). Please, contact your local Tebubio office for lot availability, specific criteria and to receive the lot-specific datasheet of your product.