Page 2 - Scientific Library
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
AIM Biochips - 3D cell culture platform for Immunotherapy and T Cell therapy studies
As mentioned in a previous post the DAX Biochips from AIM Biotech are an innovative and versatile microfluidic platform, allowing researchers to more easily develop 3D cell culture
Cancer immunotherapy: explore the cytokine "milieu" with Quantibody 200 Biomarker Service
Autologous tumour cell vaccines vs. dendritic cell vaccines: What's best, and why?
Understanding the best approach for tumour vaccinations is of growing importance in the personalized cancer immunotherapy
Multiplex profiling in one single blot: 15 species models to choose from
In a previous post, I introduced the C-series multiplex profiling technology from Raybiotech, a smart way to multiplex your western blot while saving time and sample volume, and decreasing
CAR T-Cell therapy: new hope for Immunotherapy and Cancer treatment
Personalized treatment for cancer reached a new milestone with the FDA approval of CAR T-cells for treatment of leukemia and lymphoma. There is now rapid growth in research on the therapeutic uses of
Gold standard one component TMB
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE®
Your complete tool box to facilitate your Immunotherapy research
Developing new therapeutic agents to boost the immune system and avoid the escape of diseased cells from the natural defenses is the common goal of all researchers in Immunotherapy. To greatly reduce time
MitoTools: Mitochondrial research has never been so easy
Involved in many physiological processes (apoptosis, aging, cell signalling..) and numerous diseases (cardiovascular disorders, to cancer and neuro-degenerative diseases…), Mitochondria have become more
PROTACs: A promising strategy in Drug Development
Thanks to its capability to recruit the natural protein degradation system of a cell, PROTACs (PROteolysis TArgeting Chimera) appears as a very promising
World's first 3',3'-Cyclic CAMP ELISA
The cGAS-cGAMP-STING cell signalling pathway plays a critical role in antiviral and antitumor immunity as well as mediating autoimmune responses. Dysregulation or aberrant activation of