Page 2 - Scientific Library

Following on from my previous post  in which I started to introduce a series of techniques to investigate the impact of miRNA expression on cell fate,

In this third post of the miRNA-related series, let's take a look at miRNA overexpression.

miRNA mimics

Short RNAs are very prone to enzymatic degradation. Novel chemical technologies now

Following on in our series on microRNAs (miRNA - Keys to open cell biology’s secret chamber)

Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.

miTarget databases

The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary

Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.

Basically, the technology allows you to start

As part of the innate immune response, Toll-like receptor 9 recognizes unmethylated CpG nucleotide sequences, which are very common in viruses and prokaryotes and more rare in vetebrates. Scientists routinely

Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing

Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such

CRISPR/Cas9 is relatively simple to

In March 2016, Mark J Osborn et al published in Molecular Therapy a major article for genome editing (doi:10.1038/mt.2015.197), about knock-out

Sequence engineering and chemical modification of CAS9 mRNA